Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multiple Sclerosis l: Introduction01:19

Multiple Sclerosis l: Introduction

Multiple sclerosis is a chronic autoimmune disease of the central nervous system (CNS) that affects the brain, spinal cord, and optic nerves. It is an inflammatory demyelinating disorder and a leading cause of neurological disability in young adults.EpidemiologyMS commonly begins between 20 and 40 years of age and is twice as common in women. Its exact cause remains unclear, but genetic susceptibility contributes, with higher risk in first-degree relatives and identical twins. A greater...
Nervous Tissue: Myelin01:25

Nervous Tissue: Myelin

The myelin sheath is a multilayered lipid and protein covering that insulates the axon of a neuron, enhancing the speed of nerve impulse conduction. Axons without this sheath are referred to as unmyelinated. Two types of neuroglia, Schwann cells in the peripheral nervous system (PNS) and oligodendrocytes in the central nervous system (CNS) are responsible for producing myelin sheaths.
Schwann cells begin to form myelin sheaths around axons during fetal development. They wrap around a small...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapamycin Partially Reverts Cavernoma Endothelial Cell Phenotype and, When Combined With Lapatinib, Ameliorates Chronic Lesions.

Journal of cellular and molecular medicine·2026
Same author

Workplace difficulties in early-stage relapsing-remitting multiple sclerosis with low physical disability.

Multiple sclerosis journal - experimental, translational and clinical·2026
Same author

Effects of a Mitochondrial Genetic Variant on Sevoflurane Hypersensitivity.

Anesthesiology·2026
Same author

Personalizing Relapsing-Remitting Multiple Sclerosis Monitoring: Patient Acceptance of Serum Neurofilament Light Chain and the Role of Disease Knowledge.

Journal of personalized medicine·2026
Same author

Effects of Tributyltin Chloride on Human Neuronal Differentiation and Mice Brain Development.

Environmental toxicology·2026
Same author

Mutant calreticulin enables potent and selective CAR-T cell therapy in preclinical models of myeloproliferative neoplasms.

Journal for immunotherapy of cancer·2026

Related Experiment Video

Updated: May 12, 2026

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis
09:41

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Published on: July 19, 2019

Stressed cybrids model demyelinated axons in multiple sclerosis.

Laura Llobet1, Aurora Gómez-Durán, Ruth Iceta

  • 1Departamento de Bioquímica, Biología Molecular y Celular, Centro de Investigaciones Biomédicas En Red de Enfermedades Raras (CIBERER) Universidad de Zaragoza, Zaragoza, Spain.

Metabolic Brain Disease
|April 25, 2013
PubMed
Summary

Mitochondrial DNA haplogroups may influence multiple sclerosis (MS) susceptibility. This study used cell models to show that energy stress impacts mitochondrial biogenesis, suggesting potential new therapies for MS.

More Related Videos

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
09:46

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Published on: September 21, 2021

Related Experiment Videos

Last Updated: May 12, 2026

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis
09:41

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

Published on: July 19, 2019

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
09:46

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Published on: September 21, 2021

Area of Science:

  • Neuroscience
  • Genetics
  • Cell Biology

Background:

  • Multiple sclerosis (MS) arises from complex genetic and environmental interactions.
  • Mitochondrial DNA (mtDNA) genetic backgrounds are implicated in MS pathogenesis.
  • Understanding mtDNA haplogroup effects in controlled settings is crucial for MS research.

Purpose of the Study:

  • To investigate the role of mtDNA haplogroups in MS by creating and analyzing human transmitochondrial cell lines.
  • To simulate axon demyelination, a key feature of MS pathology, within these cell models.
  • To assess the impact of simulated demyelination on mitochondrial function and biogenesis.

Main Methods:

  • Construction of human transmitochondrial cell lines (cybrids) with different mtDNA backgrounds.
  • Simulation of axon demyelination by manipulating ionic gradients and increasing cellular ATP consumption.
  • Analysis of mitochondrial biogenesis and oxidative phosphorylation capacity in response to simulated stress.

Main Results:

  • Mitochondrial biogenesis was observed in the cell model, indicating cellular adaptation to energy stress.
  • UK cybrids exhibited greater mitochondrial biogenesis, correlating with lower basal oxidative phosphorylation capacity.
  • The model successfully replicated cellular processes seen in MS patients and animal demyelination models.

Conclusions:

  • The developed cell model is suitable for analyzing the contribution of specific mtDNA genotypes to MS.
  • Certain mtDNA genotypes (e.g., UK) may confer increased susceptibility to MS under energy-demanding conditions.
  • Targeting mitochondrial biogenesis pharmacologically presents a potential therapeutic strategy for multiple sclerosis.