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Updated: May 12, 2026
Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy
Published on: April 27, 2021
CellAging: a tool to study segregation and partitioning in division in cell lineages of Escherichia coli
Antti Häkkinen1, Anantha-Barathi Muthukrishnan, Andre Mora
1Department of Signal Processing, Tampere University of Technology, 33101 Tampere, Finland.
Motivation:
Cell division in Escherichia coli is morphologically symmetric. However, as unwanted protein aggregates are segregated to the cell poles and, after divisions, accumulate at older poles, generate asymmetries in sister cells' vitality. Novel single-molecule detection techniques allow observing aging-related processes in vivo, over multiple generations, informing on the underlying mechanisms.
Results:
CellAging is a tool to automatically extract information on polar segregation and partitioning in division of aggregates in E.coli, and on cellular vitality. From time-lapse, parallel brightfield and fluorescence microscopy images, it performs cell segmentation, alignment of brightfield and fluorescence images, lineage construction and pole age determination, and it computes aging-related features. We exemplify its use by analyzing spatial distributions of fluorescent protein aggregates from images of cells across generations.
Availability:
CellAging, instructions and an example are available at http://www.cs.tut.fi/%7esanchesr/cellaging/.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
