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Highly sensitive multiplexed DNA detection using multi-walled carbon nanotube-based multicolor nanobeacon.

Jia Chen1, Yong Huang, Ming Shi

  • 1Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.

Talanta
|April 27, 2013
PubMed
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Researchers developed a novel multi-walled carbon nanotube (MWCNT)-based fluorescent nanobeacon for sensitive DNA detection. This method enables precise multiplexed analysis and single nucleotide polymorphism discrimination in solution.

Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Fluorescence quenching assays are crucial for sensitive biomolecule detection.
  • Multiplexed analysis requires selective and sensitive detection methods.
  • Multi-walled carbon nanotubes (MWCNTs) offer unique optical properties for biosensing applications.

Purpose of the Study:

  • To develop a highly sensitive and selective multi-walled carbon nanotube (MWCNT)-based multicolor fluorescent nanobeacon.
  • To enable multiplexed analysis of DNA in homogeneous solution.
  • To demonstrate the method's ability to discriminate single nucleotide polymorphisms.

Main Methods:

  • Adsorption of three different dye-labeled DNA hairpins onto MWCNTs surface via π-stacking.
  • Utilizing fluorescence quenching of dyes by MWCNTs.

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  • Inducing fluorescence recovery upon hybridization with target DNA sequences.
  • Employing 15-mer DNA fragments as proof-of-principle analytes.

    • Main Results:

    • Achieved limits of detection in the range of 35-42 pM for DNA analysis.
    • Demonstrated good analytical performance for multiplexed detection.
    • Exhibited excellent ability to discriminate between single nucleotide polymorphisms (SNPs).

    Conclusions:

    • The developed MWCNT-based fluorescent nanobeacon is a promising tool for sensitive and selective multiplexed DNA analysis.
    • The method offers a robust platform for SNP detection.
    • This approach advances homogeneous solution-based DNA detection technologies.