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Disposable biosensor based on platinum nanoparticles-reduced graphene oxide-laccase biocomposite for the
Sandra A V Eremia1, Ioana Vasilescu, Antonio Radoi
1Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, Bucharest, Romania.
Abstract:
A disposable amperometric biosensor was developed for the detection of total polyphenolic compounds from tea infusions. The biosensor was designed by modifying the surface of a carbon screen-printed electrode with platinum nanoparticles and reduced graphene oxide, followed by the laccase drop-casting and stabilization in neutralised 1% Nafion solution. The obtained biosensor was investigated by scanning electron microscopy and electrochemical techniques. It was observed that platinum nanoparticles-reduced graphene oxide composite had synergistic effects on the electron transfer and increased the electroactive surface area of the carbon screen-printed electrode. The constructed analytical tool showed a good linearity in the range 0.2-2 μM for caffeic acid and a limit of detection of 0.09 μM. The value of Michaelis-Menten apparent constant was calculated from the electrochemical version of Lineweaver-Burk equation to be 2.75 μM. This disposable laccase biosensor could be a valuable tool for the estimation of total polyphenolic content from tea infusions.
