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Published on: June 21, 2024
[Cloning of Blastocystis sp subtype 3 small-subunit ribosomal DNA]
Çağrı Şakalar1, Yunus Uyar, Mehmet Ali Yürürdurmaz
1Erciyes Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Kayseri, Türkiye.
Objective:
Different sub-types or genetic variations of Blastocystis sp. are thought to play a role in the differential symptoms caused by the parasite or asymptomatic cases. In this study, it was aimed to clone a fragment of SSUrDNA gene of Blastocystis from a patient in order to define its phylogenetic subtype.
Methods:
In this study, DNA isolation from the stool of a Blastocystis infected patient was performed. Blastocystis specific primers were used to amplify a SSUrDNA genomic fragment. The amplified DNA fragment was cloned into a plasmid and sequenced using plasmid specific primers. The obtained DNA sequence was analyzed using BLAST and a phylogenetic tree was constructed using the software MEGA.
Results:
It was found that the Blastocystis isolate in our study is subtype 3.
Conclusion:
Cloning and sequencing of the target genomic region is suggested for phylogenetic analysis studies.
