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Updated: May 11, 2026

Microbead Implantation in the Zebrafish Embryo

Microbead Implantation in the Zebrafish Embryo

Published on: July 30, 2015

Retinoic acid receptors' expression and function during zebrafish early development.

Eva Oliveira¹, Marta Casado, Demetrio Raldúa

¹Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona 18, 08034 Barcelona, Spain; CESAM & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.

The Journal of Steroid Biochemistry and Molecular Biology

|April 27, 2013

Summary

Zebrafish embryos exhibit high sensitivity to retinoic acid (RA) disruptions. Early exposure to exogenous RA alters gene expression, advancing development without visible changes, highlighting risks to the retinoic acid receptor (RAR)/retinoid-X receptor (RXR) system.

Keywords:

Danio rerio Embryo Endocrine disruption Hormone receptors Microarrays Retinoids

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Published on: July 17, 2014

Area of Science:

Developmental Biology
Molecular Endocrinology
Genomics

Background:

Retinoic acid (RA) is a crucial regulator of numerous developmental processes.
RA exerts its effects by binding to nuclear receptors: retinoic acid receptor (RAR) and retinoid-X receptor (RXR).
The RAR/RXR system's role in early zebrafish development requires detailed investigation.

Purpose of the Study:

To analyze the RAR/RXR regulatory system in zebrafish during the initial 5 days of development.
To investigate the impact of exogenous retinoic acid on gene expression and developmental timing.

Main Methods:

Analysis of relative transcript abundances for zebrafish RAR and RXR genes.
Assessment of known RAR-responsive genes following exogenous RA treatment.
Transcriptomic analysis to evaluate RA's effects on endogenous RA synthesis and developmental genes.

Main Results:

A shift from maternal to embryonic transcripts occurred within the first 24 hours post-fertilization.
Exogenous RA treatment induced negative feedback on RA synthesis genes and reduced developmental transcripts.
RA exposure accelerated developmental gene expression and inhibited other development-related genes, even at non-morphologically disruptive concentrations.

Conclusions:

Zebrafish embryos display significant sensitivity to disruptions in the RAR/RXR regulatory system.
Exogenous RA can advance the developmental program by altering gene expression patterns.
The RAR/RXR system is a critical target for potential developmental disruptors in zebrafish.