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Minimally invasive bipolar fractional radiofrequency treatment upregulates anti-senescence pathways
Sachin Rangarajan1, Apexa Trivedi, Anan Abu Ubeid
1University of California Berkeley, Berkeley, California, USA.
Background:
The sirtuin gene family has been implicated in various anti-senescence pathways. Its connection, if any, with the skin wound healing response has yet to be elucidated.
Objective:
The goal of our study was to better understand the effects of FRF treatment on the sirtuin anti-senescence pathway in skin.
Methods:
Human abdominal skin was treated with FRF, and then harvested at 0, 2, 14, and 28 days post-treatment to assess for temporal changes in gene expression levels.
Results:
Decreased levels of SIRT1, 3, 5, and 7 were observed immediately post-FRF treatment. By Day 2, SIRT1, 6, and 7 expressions increased 50-100%. SIRT6 and 7 expression continued to increase through Day 28. Expression levels of apoptosis genes FoxO3 and p53 decreased, while Bax levels increased by Day 28.
Conclusions:
Our results raise the possibility that sirtuin activity may be used as an accurate corollary to clinical improvement in skin quality.
Insights
Fibroblast-derived factors (FRF) treatment impacts sirtuin gene expression in skin, potentially correlating with improved skin quality and anti-senescence effects.
Area of Science:
- Dermatology
- Molecular Biology
- Aging Research
Background:
- The sirtuin gene family plays a role in anti-senescence pathways.
- The relationship between sirtuins and skin wound healing is not well understood.
Purpose of the Study:
- To investigate the effects of fibroblast-derived factors (FRF) on the sirtuin anti-senescence pathway in human skin.
- To elucidate the temporal changes in sirtuin gene expression following FRF treatment.
Main Methods:
- Human abdominal skin samples were treated with FRF.
- Gene expression levels of sirtuins and apoptosis-related genes were analyzed at 0, 2, 14, and 28 days post-treatment.
Main Results:
- FRF treatment initially decreased SIRT1, 3, 5, and 7 expression.
- SIRT1, 6, and 7 expression significantly increased by Day 2 and continued to rise through Day 28.
- Apoptosis gene expression showed decreased FoxO3 and p53, with increased Bax by Day 28.
Conclusions:
- Sirtuin activity modulation by FRF suggests a potential link to skin quality improvement.
- Sirtuin expression levels may serve as a biomarker for clinical skin healing and rejuvenation.