Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 11, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Automated regenerable microarray-based immunoassay for rapid parallel quantification of mycotoxins in cereals.

S Oswald¹, X Y Z Karsunke, R Dietrich

¹Institute of Hydrochemistry and Chair of Analytical Chemistry, Technische Universität München, Munich, Germany.

Analytical and Bioanalytical Chemistry

|April 27, 2013

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intracerebral gadolinium deposition following blood-brain barrier disturbance in two different mouse models.

Scientific reports·2023

Same author

Prophylactic salpingectomy for prevention of ovarian cancer at the time of elective laparoscopic cholecystectomy.

The British journal of surgery·2020

Same author

Shiga toxin-producing Escherichia coli (STEC) shedding in a wild roe deer population.

Veterinary microbiology·2019

Same author

CRISPR-Cas9: A New Addition to the Drug Metabolism and Disposition Tool Box.

Drug metabolism and disposition: the biological fate of chemicals·2018

Same author

Intestinal and hepatic contributions to the pharmacokinetic interaction between gamithromycin and rifampicin after single-dose and multiple-dose administration in healthy foals.

Equine veterinary journal·2017

Same author

Correction: Composition-dependent charge transfer and phase separation in the V<sub>1-x</sub>Re<sub>x</sub>O<sub>2</sub> solid solution.

Dalton transactions (Cambridge, England : 2003)·2017

A new automated immunoassay on a reusable biochip platform enables rapid, parallel detection of multiple mycotoxins in cereals. This 19-minute method offers a reliable alternative for food safety analysis.

Area of Science:

Analytical Chemistry
Food Science
Biotechnology

Background:

Mycotoxin contamination in cereals poses a significant risk to food safety and human health.
Accurate and rapid detection methods are crucial for regulatory compliance and risk management.
Existing methods can be time-consuming and require specialized equipment.

Purpose of the Study:

To develop and evaluate an automated flow-through multi-mycotoxin immunoassay.
To utilize a novel microarray and chemiluminescence detection system for parallel mycotoxin analysis.
To assess the method's performance in cereal matrices within a short timeframe.

Main Methods:

Development of a reusable biochip with immobilized analytes on functionalized glass slides.
Implementation of a competitive indirect immunoassay format using chemiluminescence readout.

More Related Videos

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Related Experiment Videos

Last Updated: May 11, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Automated sample processing including extraction, filtration, dilution, and immunodetection.

Main Results:

Achieved parallel detection of aflatoxins, ochratoxin A, deoxynivalenol, and fumonisins in 19 minutes.
Demonstrated biochip reusability for at least 50 cycles.
Obtained good agreement with established methods (ELISA, LC-MS) for naturally contaminated samples.

Conclusions:

The developed automated immunoassay provides a fast, reliable, and reusable tool for multi-mycotoxin detection in cereals.
This technology can support efficient food safety monitoring and regulatory control.
Oat extract proved suitable as a matrix for standard preparation across various cereal types.