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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence
Samuel C Reddington1, Pierre J Rizkallah, Peter D Watson
1School of Biosciences, Cardiff University, Cardiff, UK.
Angewandte Chemie (International Ed. in English)
|April 27, 2013
Abstract:
Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

