Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D pathology-guided microdissection.

Nature methods·2026
Same author

Assessing the Effects of a 3-Dimensional (3D) Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

A systematic comparison of cavitation regimes and histotripsy efficiency across pulse duration and repetition rate in a fibrous tissue mimicking phantom.

Research square·2026
Same author

Artificial Intelligence-informed Architectural Insights of 3-dimensional Glandular Networks Identify Patients With Prostate Cancer at a Higher Risk of Biochemical Recurrence.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
Same author

High-throughput, organ-scale 3D tubule tracking using TubuleMAP.

bioRxiv : the preprint server for biology·2026
Same author

Engineered zinc finger repressors induce a prolonged and selective repression of <i>SCN9A</i> in nociceptors of nonhuman primates.

Science translational medicine·2026

Related Experiment Video

Updated: May 11, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Method for assessing the reliability of molecular diagnostics based on multiplexed SERS-coded nanoparticles.

Steven Y Leigh1, Madhura Som, Jonathan T C Liu

  • 1Stony Brook University (SUNY), Department of Biomedical Engineering, Stony Brook, New York, USA.

Plos One
|April 27, 2013
PubMed
Summary

A new spectral reliability index (SRI) helps ensure accurate results from surface-enhanced Raman scattering (SERS) nanoparticles used in molecular diagnostics. This metric improves the dependability of multiplexed nanoparticle imaging for biomedical applications.

More Related Videos

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Related Experiment Videos

Last Updated: May 11, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

  • Nanotechnology
  • Biomedical Diagnostics
  • Spectroscopy

Background:

  • Surface-enhanced Raman scattering (SERS) nanoparticles offer unique spectral fingerprints for molecular diagnostics.
  • Multiplexed SERS nanoparticles can target diverse biomarkers for enhanced biomedical imaging.
  • Background noise and detector limitations can compromise the accuracy of SERS-based diagnostics.

Purpose of the Study:

  • To develop a metric for quantifying the reliability of SERS nanoparticle spectral demultiplexing.
  • To ensure accuracy and reproducibility in time-constrained in vivo imaging experiments.
  • To provide experimentalists with a tool for monitoring diagnostic performance.

Main Methods:

  • Development of a spectral reliability index (SRI).
  • Utilizing a direct classical least-squares (DCLS) demultiplexing routine.
  • Validation through simulations and experimental data.

Main Results:

  • The proposed SRI effectively measures the reliability of computed nanoparticle concentrations and ratios.
  • Demonstrated feasibility of the SRI for improving diagnostic accuracy.
  • The SRI can be monitored to enforce accuracy bounds at a user-defined confidence level.

Conclusions:

  • The spectral reliability index (SRI) is a valuable tool for enhancing the dependability of multiplexed SERS nanoparticle applications.
  • This metric can improve the accuracy and reproducibility of in vivo molecular diagnostics.
  • The SRI strategy is adaptable for various instruments and biomedical imaging scenarios.