Related Experiment Video
Updated: May 11, 2026
03:53
Extracellular Vesicle Tissue Factor Activity Assay
Published on: December 29, 2023
Analysis of tissue factor expression in various cell model systems: cryptic vs. active
H Kothari1, U R Pendurthi, L V M Rao
1Department of Cellular and Molecular Biology, Center for Biomedical Research, The University of Texas Health Science Center at Tyler, TX 75708, USA.
Journal of Thrombosis and Haemostasis : JTH
|April 30, 2013
Summary
Tissue factor (TF) encryption is common across cell types, with most TF existing in an inactive, cryptic form. Cell activation increases TF activity by converting cryptic TF to its active state, not by enhancing existing active TF.
Area of Science:
- Biochemistry
- Cell Biology
- Hematology
Background:
- Tissue factor (TF) encryption regulates its coagulant activity.
- Understanding TF encryption mechanisms has been hindered by varied experimental models.
Purpose of the Study:
- Characterize TF procoagulant activity across different cell types.
- Determine if increased TF activity upon activation results from cryptic TF transformation.
Main Methods:
- Kinetic analyses of TF-FVIIa activation and FVIIa binding.
- Utilized fibroblast, cancer, endothelial, and monocytic cell models.
- Estimated TF coagulant-specific activity and cryptic/active TF percentages.
Main Results:
- MDA-231 and WI-38 cells express significantly more TF than HUVEC and THP-1 cells.
- TF-specific activity was similar across MDA-231, WI-38, and THP-1 cells.
- 80-90% of TF in MDA-231, WI-38, and THP-1 cells was cryptic; activation increases activity via decryption.
Conclusions:
- TF encryption is not cell-type specific.
- The majority of TF in cancer cells is not constitutively procoagulant.
- Cell activation enhances TF procoagulant activity through decryption of cryptic TF.
