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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Reporter mouse lines for fluorescence imaging.

Takaya Abe1, Toshihiko Fujimori

  • 1Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.

Development, Growth & Differentiation
|April 30, 2013
PubMed
Summary

Researchers need better tools for live imaging in mouse development. This review details available reporter mouse lines, aiding scientists in selecting the best options for their studies.

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Area of Science:

  • Developmental biology
  • Genetics
  • Microscopy

Background:

  • Live imaging is crucial for understanding dynamic processes in mouse development.
  • Numerous fluorescent reporter mouse lines exist, but a centralized database is lacking.
  • Researchers face challenges in identifying suitable reporter lines for specific live imaging needs.

Purpose of the Study:

  • To review and summarize existing reporter mouse lines for live imaging.
  • To discuss the characteristics of these mouse lines.
  • To aid researchers in selecting appropriate reporter lines.

Main Methods:

  • Literature review of published reporter mouse lines.
  • Analysis of reporter mouse line characteristics for live imaging applications.
  • Discussion of Cre transgenic lines (Cre-X-Mice) and other reporter systems.

Main Results:

  • Compilation of various reporter mouse lines suitable for live imaging.
  • Detailed discussion on the features and applications of these lines.
  • Identification of gaps in current resources for reporter mouse line selection.

Conclusions:

  • A comprehensive understanding of available reporter mouse lines is essential for advancing live imaging studies in mouse development.
  • Standardization and accessibility of information on reporter lines would benefit the research community.
  • Further development of reporter systems can enhance the study of developmental dynamics.