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Related Experiment Video

Updated: May 11, 2026

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

Development of dextran nanoparticles for stabilizing delicate proteins.

Fei Wu1, Zhihua Zhou, Jing Su

  • 1School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. lmwei@sjtu.edu.cn.

Nanoscale Research Letters
|April 30, 2013
PubMed
Summary

This study introduces novel dextran nanoparticles for stabilizing and encapsulating proteins, preserving their bioactivity. These nanoparticles effectively protect delicate proteins from degradation and harsh microenvironments during formulation and administration.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Pharmaceutical Sciences

Background:

  • Protein pharmaceuticals face stability challenges due to complex structures.
  • Developing effective delivery systems is crucial for protein drug efficacy.

Purpose of the Study:

  • To develop a novel method for stabilizing and encapsulating proteins into dextran nanoparticles.
  • To ensure protein bioactivity is maintained throughout the formulation process.

Main Methods:

  • Proteins were encapsulated into dextran nanoparticles using aqueous-aqueous freezing-induced phase separation.
  • Encapsulation efficiency, recovery, particle characteristics, protein aggregation, and bioactivity were assessed.
  • Dextran nanoparticles' ability to resist acidic microenvironments was evaluated using a pH-sensitive fluorescent indicator.

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Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
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Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

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Last Updated: May 11, 2026

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

Main Results:

  • The novel dextran nanoparticles successfully encapsulated various model proteins (BSA, GM-CSF, G-CSF, β-galactosidase, myoglobin).
  • Protein aggregation was minimized, and bioactivity of encapsulated proteins (GM-CSF, G-CSF, β-galactosidase) was preserved.
  • Dextran nanoparticles demonstrated the ability to attenuate acidic microenvironments, protecting encapsulated payloads.

Conclusions:

  • This novel dextran nanoparticle formulation offers an effective approach for stabilizing delicate proteins.
  • The method preserves protein bioactivity during preparation and protects against harsh conditions.
  • These nanoparticles present a promising strategy for protein drug delivery and administration.