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Related Experiment Video

Updated: May 11, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

eIF3c: a potential therapeutic target for cancer.

Rafi Emmanuel¹, Shiri Weinstein, Dalit Landesman-Milo

¹Laboratory of Nanomedicine, Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

|April 30, 2013

Summary

Targeting the housekeeping gene eIF3c, vital for protein synthesis, shows therapeutic potential against diverse cancers. RNA interference (RNAi) against eIF3c effectively reduced cancer cell proliferation and induced cell death, highlighting its broad applicability.

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Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

Area of Science:

Molecular Biology
Cancer Research
Nanomedicine

Background:

Cancer cells exhibit genomic instability, leading to heterogeneity and therapeutic challenges.
Targeting ubiquitously expressed housekeeping genes offers a potential strategy against diverse malignancies.

Purpose of the Study:

To investigate the therapeutic potential of the housekeeping gene eIF3c in various cancer cell lines.
To evaluate the efficacy of targeting eIF3c using RNA interference (RNAi) approaches.

Main Methods:

RNAi-mediated downregulation of eIF3c in five cancer cell lines (NCI-ADR/RES, HeLa, MCF7, HCT116, B16F10).
Assessment of global protein synthesis, cell cycle arrest, proliferation, and cell death.
Utilized hyaluronan (HA)-coated lipid-based nanoparticles (LNPs) for siRNA delivery in mouse melanoma cells.

Main Results:

eIF3c downregulation significantly decreased global protein synthesis and induced polysome run-off.
Reduced eIF3c expression led to cell cycle arrest (G0/G1 or G2/M) in a tissue-dependent manner.
This resulted in decreased cell proliferation and induced cancer cell death, with successful siRNA delivery via HA-coated LNPs.

Conclusions:

The housekeeping gene eIF3c is crucial for translation initiation and cancer cell survival.
Targeting eIF3c presents a promising therapeutic strategy for heterogeneous malignancies.
Ubiquitously expressed genes like eIF3c are more effective targets than tumor-specific overexpressed genes.