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Updated: May 11, 2026

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
06:21

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells

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KSHV ORF67 encoded lytic protein localizes on the nuclear membrane and alters emerin distribution

Antonella Farina1, Roberta Santarelli, Rossella Bloise

  • 1Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Medicina Sperimentale, Sapienza Università di Roma, Italy. alberto.faggioni@uniroma1.it

Virus Research
|April 30, 2013
PubMed

Insights

Kaposi

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Kaposi's sarcoma-associated herpesvirus (KSHV) is a human oncogenic virus.
  • Viral protein p29 (ORF67) is a homolog of conserved herpesvirus protein UL34.
  • p29 is expressed early in the viral lytic cycle and localizes to the nuclear rim.

Purpose of the Study:

  • To investigate the function of KSHV protein p29 during viral replication.
  • To determine the interaction of p29 with other viral proteins and host cell components.
  • To elucidate the role of p29 in nuclear lamina disassembly.

Main Methods:

  • Immunofluorescence assay (IFA) and biochemical analysis were used.
  • Cells were infected or transfected with KSHV or its genes.
  • Expression and localization of viral and host proteins were analyzed.

Main Results:

  • KSHV p29 interacts with p33 (ORF69) and they colocalize on the nuclear membrane.
  • p29 expression causes delocalization and hyperphosphorylation of emerin.
  • Combined expression of p29 and p33 enhances emerin mislocalization.

Conclusions:

  • Emerin destabilization by p29 and p33 may initiate nuclear lamina disassembly.
  • This process is crucial for KSHV nucleocapsid maturation.
  • p29 plays a significant role in KSHV replication and pathogenesis.

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