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Related Concept Videos

Redox Reactions01:24

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Redox Titration: Overview01:21

Redox Titration: Overview

Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...

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Related Experiment Video

Updated: May 11, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

Gel-based methods in redox proteomics.

Rebecca Charles1, Tamani Jayawardhana, Philip Eaton

  • 1King's College London, Cardiovascular Division, The British Heart Foundation Centre of Excellence, The Rayne Institute, St Thomas' Hospital, London SE1 7EH, UK.

Biochimica Et Biophysica Acta
|April 30, 2013
PubMed
Summary

Developing tools to study protein thiol oxidative modifications is crucial for understanding oxidants in health and disease. Gel-based methods help detect, quantify, and identify these critical redox sensor proteins.

Keywords:
Redox proteomics

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Last Updated: May 11, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Published on: June 18, 2020

Area of Science:

  • Biochemistry
  • Cellular Biology
  • Oxidative Stress Research

Background:

  • Cellular redox balance is critical for health and disease.
  • Oxidant-reactive deprotonated thiols in proteins act as key redox sensors.
  • Monitoring oxidative modifications of these thiols requires specialized tools.

Purpose of the Study:

  • To review gel-based methods for detecting, quantifying, and identifying oxidative modifications in protein thiols.
  • To categorize techniques based on their approach to monitoring thiol oxidation.
  • To highlight the importance of understanding thiol redox sensing in biological systems.

Main Methods:

  • Discussion of gel-based techniques for monitoring thiol oxidation.
  • Categorization into methods detecting global or specific thiol oxidation.
  • Utilizing thiol-labeling reagents to track oxidative modifications.

Main Results:

  • A range of gel-based methods are available for studying thiol redox modifications.
  • These methods enable the detection and quantification of thiol redox states.
  • Specific oxidation states like S-sulfenylation and S-nitrosylation can be investigated.

Conclusions:

  • Gel-based methods are valuable for identifying thiol proteins sensitive to oxidative modifications.
  • These techniques aid in the detection, quantification, and identification of thiol redox sensors.
  • Understanding protein thiol redox sensing enhances appreciation of oxidants' roles in health and disease.