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Manufacture and Drug Delivery Applications of Silk Nanoparticles

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

Pullulan-based nanoparticles as carriers for transmucosal protein delivery.

Marita Dionísio¹, Clara Cordeiro, Carmen Remuñán-López

¹CBME - Centre for Molecular and Structural Biomedicine/IBB - Institute for Biotechnology and Bioengineering, 8005-139 Faro, Portugal. maritadionisio@gmail.com

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences

|April 30, 2013

Summary

Pullulan-based nanoparticles were developed using a mild polyelectrolyte complexation method for effective protein delivery. These biocompatible nanoparticles showed no significant toxicity in a respiratory cell model, indicating their potential for transmucosal protein therapeutics.

Keywords:

Carrageenan Chitosan Drug delivery Nanoparticles Protein delivery Pullulan

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Area of Science:

Biomaterials Science
Nanotechnology
Drug Delivery

Background:

Polymeric nanoparticles are effective for transmucosal protein delivery.
Polysaccharides offer biocompatibility and biodegradability for nanocarrier production.
Mild production methods are preferred to prevent protein degradation and facilitate scale-up.

Purpose of the Study:

To develop pullulan-based nanoparticles using a mild polyelectrolyte complexation method.
To functionalize pullulan with charge via sulfation and amination for complexation.
To evaluate the protein association, release kinetics, stability, and cytotoxicity of the developed nanoparticles.

Main Methods:

Synthesis of sulfated and aminated pullulan derivatives.
Polyelectrolyte complexation of pullulan derivatives with chitosan and carrageenan.
Characterization of nanoparticle size, zeta potential, and protein association.
In vitro protein release studies in PBS pH 7.4.
Assessment of nanoparticle stability upon freeze-drying.
Cytotoxicity evaluation using MTT assay on Calu-3 respiratory cell model.

Main Results:

Positively charged pullulan-based nanoparticles (180-270 nm) were successfully produced.
Nanoparticles effectively associated with bovine serum albumin (model protein).
A 30% burst release of protein was observed, followed by sustained release up to 24 hours.
Nanoparticle size and zeta potential remained stable after freeze-drying with cryoprotectants.
No overt toxicity was observed in the Calu-3 respiratory cell model.

Conclusions:

Pullulan-based nanoparticles can be produced via a mild, scalable polyelectrolyte complexation method.
These nanoparticles demonstrate potential for transmucosal protein delivery applications.
The developed nanocarriers exhibit good biocompatibility and stability, making them suitable for therapeutic protein encapsulation.