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Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

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Published on: February 20, 2013

iTRAQ-labeling for biomarker discovery.

Leroi V Desouza¹, Sébastien N Voisin, K W Michael Siu

¹Department of Chemistry, York University, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|April 30, 2013

Summary

This summary is machine-generated.

Mass-tagging techniques like iTRAQ labeling enable protein quantification for cancer biomarker discovery. This approach aids in identifying potential biomarkers from clinical samples for further validation studies.

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Area of Science:

Proteomics
Biomarker Discovery
Mass Spectrometry

Background:

Mass-tagging techniques have advanced proteomics research, enabling relative and absolute protein quantification in complex samples.
These methods facilitate studies on proteome alterations in response to stimuli and biomarker discovery.
iTRAQ labeling is a specific mass-tagging approach used in proteomics.

Purpose of the Study:

To describe the application of iTRAQ labeling for cancer biomarker discovery.
To highlight the utility of mass-tagging in identifying potential cancer biomarkers.

Main Methods:

Utilized iTRAQ (isobaric Tandem Mass Spectrophilicity Reporter) labeling, a mass-tagging technique.
Applied the method to analyze tens of clinical samples for cancer biomarker discovery.
Employed mass spectrometry for protein quantification.

Main Results:

The study demonstrates the feasibility of using iTRAQ labeling for cancer biomarker discovery.
Analysis of clinical samples provided initial leads for potential biomarkers.
This approach can generate valuable data for subsequent validation studies.

Conclusions:

iTRAQ labeling is a valuable tool for proteomics-based cancer biomarker discovery.
Mass-tagging technologies offer a pathway for identifying potential biomarkers from clinical cohorts.
The findings support the use of iTRAQ for generating hypotheses for targeted validation studies.