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Related Experiment Video

Updated: May 11, 2026

Manufacture and Drug Delivery Applications of Silk Nanoparticles
09:03

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

pH-dependent anticancer drug release from silk nanoparticles.

F Philipp Seib1, Gregory T Jones, Jelena Rnjak-Kovacina

  • 1Tufts University, Department of Biomedical Engineering, 4 Colby Street, Medford, MA 02155, USA.

Advanced Healthcare Materials
|April 30, 2013
PubMed
Summary
This summary is machine-generated.

Silk nanoparticles offer a novel approach to cancer treatment, demonstrating pH-dependent doxorubicin release and overcoming drug resistance. These biocompatible nanoparticles accumulate in cancer cells, paving the way for advanced nanomedicine therapies.

Keywords:
adriamycinbombyx morifibroinnanomedicine

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Last Updated: May 11, 2026

Manufacture and Drug Delivery Applications of Silk Nanoparticles
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Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery
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Published on: June 23, 2020

Area of Science:

  • Biomaterials Science
  • Nanomedicine
  • Oncology

Background:

  • Silk's traditional use in sutures highlights its mechanical strength and biocompatibility.
  • Silk-based materials are explored for tissue engineering and regenerative medicine.
  • Limited research exists on silk particles for drug delivery, particularly as stimulus-responsive anticancer nanomedicine.

Purpose of the Study:

  • To develop and characterize silk nanoparticles for potential use as a stimulus-responsive anticancer nanomedicine.
  • To evaluate the doxorubicin loading capacity and pH-dependent release profile of silk nanoparticles.
  • To assess the in vitro cytotoxicity and efficacy of doxorubicin-loaded silk nanoparticles against human breast cancer cell lines.

Main Methods:

  • Acetone precipitation was used to synthesize uniform silk nanoparticles (98 nm diameter).
  • Doxorubicin loading and pH-dependent release kinetics were quantified.
  • In vitro cytotoxicity assays (IC50) and live cell fluorescence microscopy were performed on human breast cancer cell lines.

Main Results:

  • Uniform silk nanoparticles with negative surface charge were synthesized in a single step.
  • Silk nanoparticles efficiently loaded doxorubicin with significant pH-dependent release (pH 4.5 > 6.0 > 7.4).
  • Doxorubicin-loaded silk nanoparticles showed no cytotoxicity, overcame drug resistance, and were taken up by cancer cells via endocytosis, accumulating in lysosomes.

Conclusions:

  • Silk nanoparticles are a promising platform for developing stimulus-responsive anticancer nanomedicine.
  • The pH-dependent drug release and lysosomal accumulation support their potential as lysosomotropic anticancer agents.
  • Silk nanoparticles demonstrate efficacy against drug-resistant cancer cells, offering a new therapeutic avenue.