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Related Experiment Video

Updated: May 11, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

ABCC6 is a basolateral plasma membrane protein.

Viola Pomozi¹, Olivier Le Saux, Christopher Brampton

¹Institute of Enzymology, RCNS, Hungarian Academy of Sciences, Budapest, Hungary.

Circulation Research

|April 30, 2013

Summary

This summary is machine-generated.

The ATP-binding cassette C6 (ABCC6) protein is located in the basolateral membrane of liver cells, not mitochondria. This finding clarifies ABCC6

Keywords:

arterial calcification, generalized, of infancy hepatocytes pseudoxanthoma elasticum soft tissue calcification

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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Area of Science:

Cell Biology
Molecular Medicine
Biochemistry

Background:

The ATP-binding cassette C6 (ABCC6) protein is critical for preventing ectopic calcification.
ABCC6 gene mutations are linked to pseudoxanthoma elasticum and infantile arterial calcification.
Understanding ABCC6's precise cellular location is vital for disease research.

Purpose of the Study:

To determine the correct subcellular localization of the native ABCC6 protein.
To resolve conflicting reports regarding ABCC6 localization (plasma membrane vs. mitochondria-associated membrane).

Main Methods:

Immunofluorescent labeling of mouse and human liver tissue sections.
Analysis of primary hepatocytes.
Utilized multiple antibodies targeting human and mouse ABCC6.

Main Results:

ABCC6 is unequivocally localized to the basolateral membrane of hepatocytes.
ABCC6 shows no association with mitochondria, mitochondria-associated membranes, or the endoplasmic reticulum.
Results were consistent across different tissue types and antibodies.

Conclusions:

ABCC6 resides in the basolateral membrane of hepatocytes.
This localization supports a role for ABCC6 in exporting metabolites from hepatocytes into circulation.