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Updated: May 11, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
ValiDichro: a website for validating and quality control of protein circular dichroism spectra
Benjamin Woollett1, Lee Whitmore, Robert W Janes
1Institute of Structural and Molecular Biology, Birkbeck College, University of London, London WC1E 7HX, UK.
None:
Circular dichroism (CD) spectroscopy is widely used in structural biology as a technique for examining the structure, folding and conformational changes of proteins. A new server, ValiDichro, has been developed for checking the quality and validity of CD spectral data and metadata, both as an aid to data collection and processing and as a validation procedure for spectra to be included in publications. ValiDichro currently includes 25 tests for data completeness, consistency and quality. For each test that is done, not only is a validation report produced, but the user is also provided with suggestions for correcting or improving the data. The ValiDichro server is freely available at http://valispec.cryst.bbk.ac.uk/circularDichroism/ValiDichro/upload.html.
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