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The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

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Syntaxin 16 regulates lumen formation during epithelial morphogenesis.

Jae-Joon Jung¹, Shivangi M Inamdar, Ajit Tiwari

¹Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa, United States of America.

|April 30, 2013

Summary

This summary is machine-generated.

Syntaxin 16 (Stx16) is crucial for kidney epithelial lumen development. Depleting Stx16 disrupts cell junctions and leads to abnormal lumen formation in vitro and in vivo.

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Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Published on: June 27, 2016

Area of Science:

Cell Biology
Molecular Biology
Developmental Biology

Background:

Cell-cell junction formation and maintenance rely on targeted protein trafficking.
Syntaxin (Stx) proteins regulate vesicle fusion and intracellular transport.
The roles of endosome- and Golgi-localized Stx proteins in epithelial morphogenesis are not well understood.

Purpose of the Study:

To investigate the role of endosome- and Golgi-localized Stx16 in epithelial lumen development.
To determine the function of Stx16 in maintaining cell-cell junction integrity.

Main Methods:

Utilized Madin Darby Canine Kidney (MDCK) cells for in vitro studies.
Assessed Stx16 expression levels during cell confluency.
Depleted Stx16 in MDCK cells and analyzed transepithelial resistance and E-cadherin localization.
Formed MDCK cell cysts in Matrigel to observe lumen development.
Examined pronephric duct development in zebrafish morphants lacking Stx16 function.

Main Results:

Stx16 expression increased as MDCK cells reached confluency.
Stx16 depletion resulted in lower transepithelial resistance and impaired E-cadherin maintenance due to reduced recycling.
MDCK cell cysts formed by Stx16-depleted cells exhibited multiple lumens, unlike control cysts with single lumens.
Zebrafish pronephric ducts in stx16 morphants were enlarged, tortuous, and possessed multiple lumens.

Conclusions:

Stx16 is essential for proper epithelial lumen morphogenesis.
Stx16 plays a critical role in maintaining cell-cell junction integrity through protein trafficking and recycling.
Disruption of Stx16 function leads to defects in lumen formation both in vitro and in vivo.