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[Comparison of two different methods to detect HIV antibodies]
Jing-Xia Guo1, Jun Xu, Lin Chen
1Center of Clinical Laboratory, The No. 302 Hospital of the PLA, Beijing, China.
Objective:
Evaluated the chemiluminescence and enzyme-linked immunosorbent assay (ELISA) to detect HIV antibodies, and compared the results, to provide a reference for the selection and clinical application of HIV screening.
Methods:
3000 cases of our hospital patients were measured by enzyme-linked immunosorbent assay and chemiluminescence immunoassay, using comfirmming experimental results as gold standards. Comparing sensitivity, specificity and other Indicators.
Results:
In the diagnosis of HIV infection, enzyme-linked immunosorbent assay and chemiluminescence immunoassay had no significant difference. The positive rate of enzyme-linked immunosorbent assay was 0.93%, while the sensitivity and specificity were 89.66%, 99.93%, the positive rate of chemiluminescence immunoassay was 1.03%, while the sensitivity and specificity were 100%, 99.93%, respectively.
Conclusion:
Both methods are suitable for screening of HIV, having high specificity, and chemiluminescence has greater sensitivity than ELISA.
