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Updated: May 11, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Published on: March 17, 2023
[Genetic transformation of Pinellia ternata with Agrobacterium tumefaciens-mediated sHSP genes]
Zhao-Yang Guo1, Ting-Ting Cui, Jian-Ping Xue
1Anhui Key Laboratory of Plant Resources and Biology, School of Life science, Huaibei Normal University, Huaibei 235000, China.
Objective:
To establish an efficient genetic transformation system of Pinellia ternata.
Method:
With petioles from test-tube seedlings of P. ternata as explants, Agrobacterium tumefaciens mediation method was adopted to explore the effect of phenolic substances, A. tumefaciens's concentration, infection time, pre-incubation time and co-cultivation time on genetic transformation efficiency of P. ternata.
Result And Conclusion:
The genetic transformation efficiency could be effectively enhanced by infecting in A. tumefaciens culture containing AS 40 mg x L(-1) for 15 min for three days. The petioles were put into the differentiation medium containing 150 mg x L(-1) Kan and 350 mg x L(-1) Carb to screening and cultivation. After around 30 days, microtubers could be observed at both sides of the petioles. Gus staining and PCR verification on the regenerated plants showed that the exogenous gene sHSP had been integrated into genome of P. ternata.
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