Related Experiment Video
Updated: May 11, 2026
Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
Published on: May 31, 2017
[Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus]
Gui-Xiang Wan1, Lin Ma, Jian Zhang
1Tianjin University of Traditional Chiness Medicine, Tianjin 300193, China. wgx0502@126.com
Objective:
To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.
Method:
HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions: ODS column was adopted, with methanol-water-phosphate (47: 53: 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.
Result:
S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).
Conclusion:
The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.
