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Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
Published on: August 10, 2018
Bidirectional and mutually beneficial interactions between human mesenchymal stem cells and osteoarthritic
Hua Jia Diao1, Chui Wai Yeung, Chun Hoi Yan
1Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China.
Aim:
Mesenchymal stem cell (MSC)-based therapy presents a promising approach for treating osteoarthritis (OA). However, the molecular interactions between MSCs and OA chondrocytes (OACs) are not known. This study aims to investigate the bidirectional interactions between human MSCs (hMSCs) and human OACs (hOACs) in a 3D co-culture system.
Materials & Methods:
hMSC-collagen microspheres were cultured in hOAC-conditioned medium or co-cultured with hOAC-collagen microspheres. Growth characteristics, glycosaminoglycan (GAG) production, gene expression of major OA-associated chondrogenic markers, including SOX9, COL2A1, ACAN and MMP13, were investigated in both cell types.
Results:
Both the conditioned medium and the co-culture induced MSC chondrogenesis with enhanced GAG production, SOX9 gene and protein expression, and gene expression of ACAN and COL2A1. Meanwhile, the co-culture also induced hOACs to partially resume the lost chondrogenic phenotype as shown by reduced proliferation, enhanced GAG production when hMSCs were chondrogenically predifferentiated, and reduced MMP13 gene expression.
Conclusion:
This work suggests that 3D co-culture of hMSCs and hOACs is mutually beneficial to each other, suggesting the potential therapeutic effect of delivering hMSC in scaffolds directly to OA defects.
Insights
Mesenchymal stem cells (MSCs) and osteoarthritis chondrocytes (OACs) benefit each other in 3D co-culture. This suggests MSCs delivered in scaffolds may treat osteoarthritis defects effectively.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Cell Biology
Background:
- Osteoarthritis (OA) is a degenerative joint disease.
- Mesenchymal stem cell (MSC)-based therapy shows promise for OA treatment.
- Molecular interactions between MSCs and OA chondrocytes (OACs) remain unclear.
Purpose of the Study:
- Investigate bidirectional interactions between human MSCs (hMSCs) and human OACs (hOACs).
- Utilize a 3D co-culture system to model these interactions.
Main Methods:
- hMSC-collagen microspheres were cultured in hOAC-conditioned medium or co-cultured with hOAC-collagen microspheres.
- Assessed growth characteristics, glycosaminoglycan (GAG) production, and gene expression (SOX9, COL2A1, ACAN, MMP13).
Main Results:
- Co-culture and conditioned medium enhanced MSC chondrogenesis, GAG production, and expression of SOX9, ACAN, and COL2A1.
- Co-culture partially restored the chondrogenic phenotype in hOACs, reducing proliferation and MMP13 expression while enhancing GAG production.
Conclusions:
- 3D co-culture of hMSCs and hOACs demonstrates mutual benefits.
- This suggests potential therapeutic efficacy of delivering MSCs in scaffolds directly to OA defects.
