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Related Experiment Video

Updated: May 11, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Vascularization in 3D tissue using cell sheet technology.

Sachiko Sekiya1, Tatsuya Shimizu, Teruo Okano

  • 1Institute of Advanced Biomedical Engineering & Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

Regenerative Medicine
|May 1, 2013
PubMed
Summary

Tissue engineering faces diffusion limitations in creating thick 3D tissues. This study introduces a novel cell sheet technology to overcome these challenges and enable vascularized 3D tissue fabrication for regenerative medicine.

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • Tissue engineering integrates multiple scientific disciplines.
  • Current limitations in fabricating thick 3D tissues are primarily due to diffusion.
  • Vascularization is essential for advancing 3D tissue fabrication.

Purpose of the Study:

  • To address the diffusion limitation in 3D tissue engineering.
  • To introduce and evaluate a novel cell sheet technology.
  • To compare this technology with existing methods for fabricating vascularized 3D tissue.

Main Methods:

  • Development of a proprietary cell sheet technology.
  • Fabrication of vascularized 3D tissue constructs.
  • Comparative analysis of the new technology against established methods.

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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

Related Experiment Videos

Last Updated: May 11, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

Main Results:

  • The proposed cell sheet technology facilitates the fabrication of thicker 3D tissues.
  • Demonstrated successful vascularization within engineered tissue constructs.
  • Comparative data highlights the advantages of the new approach.

Conclusions:

  • The novel cell sheet technology effectively overcomes diffusion limitations in 3D tissue engineering.
  • This advancement is crucial for the development of clinically relevant vascularized tissues.
  • The technology holds significant promise for regenerative medicine applications.