Enhanced spectral analysis of blood flow during post-occlusive reactive hyperaemia test in different tissue depths

The objective of this study was to investigate the impairment of microcirculation in schizophrenic patients by means of spectral analysis of blood flow signals and to determine if microcirculation is unequally altered in different tissue depths. Furthermore, the impact of gender and age on the spectral parameters of the Laser Doppler Flowmetry (LDF) signal in healthy and diseased microcirculation are to be analysed. The segmented spectral analysis (SSA) algorithm was applied to LDF signals of a provoked post-ischemic stage and compared to the traditional total spectral analysis, hypothesizing that SSA reveals more detailed information on the dynamic behaviour of the blood flow. 15 healthy subjects (CON, mean age 32.4 years) and 15 patients (PAT, mean age 33.0 years) were enrolled. Spectral analysis was performed on two LDF signals at a depth of 2mm and 6-8mm. Features in five frequency subintervals were determined. Our results indicate that microcirculation is strongly impaired in patients. SSA of blood flow revealed differences between CON and PAT in all three frequency intervals referring to local vasomotion (endothelial p=0.03; sympathetic p=0.02, myogenic p=0.03) as well as the respiratory (p=0.02) and cardiac (p=0.006) bands in the deeper tissue. In contrast, in the near-surface tissue only the endothelial (p=0.006) and cardiac (p=0.006) components were altered. Furthermore, SSA determined a gender- and age dependency regarding blood flow. In conclusion, we could demonstrate that microcirculation in schizophrenic patients is significantly impaired, depending on its location in the near-surface skin or in the superficial muscle tissue. These alterations of microcirculation are more pronounced in the deeper tissue depth of about 6-8mm and are influenced by gender and age.