Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metal-Ligand Bonds02:51

Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Coordination Number and Geometry02:57

Coordination Number and Geometry

For transition metal complexes, the coordination number determines the geometry around the central metal ion. Table 1 compares coordination numbers to molecular geometry. The most common structures of the complexes in coordination compounds are octahedral, tetrahedral, and square planar.
Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Iron Accumulation and Metabolic Alterations in Aneurysmal Subarachnoid Hemorrhage.

Stroke (Hoboken, N.J.)·2026
Same author

Pyomo: Accidentally outrunning the bear.

Patterns (New York, N.Y.)·2025
Same author

Humanin variants aggregate to produce different fibril morphologies.

The Journal of biological chemistry·2025
Same author

AAV gene therapy in companion dogs with severe hemophilia: Real-world long-term data on immunogenicity, efficacy, and quality of life.

Molecular therapy. Methods & clinical development·2024
Same author

Conquering pressure! The effects of mild-anxiety training on motor performance under pressure during early motor learning.

Journal of experimental psychology. Human perception and performance·2024
Same author

Observation of pH-Dependent Residual Structure in the Pmel17 Repeat Domain and the Implication for Its Amyloid Formation.

Biochemistry·2023
Same journal

The chemistry of the cobalt corrinoids - Recent advances and emerging themes. Part 3. Cobalamins and health.

Journal of inorganic biochemistry·2026
Same journal

PIKfyve-specific Pt(II)-based targeted drug conjugate in treatment of ovarian cancer through multi-mode actions.

Journal of inorganic biochemistry·2026
Same journal

From PET to targeted radionuclide therapy in the Brain: The emerging role of radiometal-based platforms.

Journal of inorganic biochemistry·2026
Same journal

The chemistry of the cobalt corrinoids - Recent advances and emerging themes. Part 2. The biochemistry, microbiology, and ecology.

Journal of inorganic biochemistry·2026
Same journal

Substituent effects in picolinic acid-derived silver(I) and zinc(II) complexes: Structure, stability, DNA interactions and therapeutic potential.

Journal of inorganic biochemistry·2026
Same journal

Cadmium(II) imidazole coordination complexes as selective antifungal agents against resistant Candida: Insights into protein binding, electrochemistry, and CYP51 binding predictions.

Journal of inorganic biochemistry·2026
See all related articles

Related Experiment Video

Updated: May 11, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

The abilities of selenium dioxide and selenite ion to coordinate DNA-bound metal ions and decrease oxidative DNA

William E Hart1, Steven P Marczak, Andrew R Kneller

  • 1Department of Chemistry and Biochemistry, Rose-Hulman Institute of Technology, Terre Haute, IN 47803-3920, USA.

Journal of Inorganic Biochemistry
|May 1, 2013
PubMed
Summary
This summary is machine-generated.

Selenium compounds like SeO2 and Na2SeO3 significantly reduce metal-induced oxidative DNA damage. These selenium-metal complexes act sacrificially, protecting DNA from reactive oxygen species generated by transition metals and hydrogen peroxide.

More Related Videos

Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
09:00

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles

Published on: March 18, 2015

Related Experiment Videos

Last Updated: May 11, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
09:00

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles

Published on: March 18, 2015

Area of Science:

  • Biochemistry and Molecular Biology
  • Environmental Chemistry
  • Toxicology

Background:

  • Transition metals interacting with hydrogen peroxide (H2O2) generate reactive oxygen species (ROS), causing oxidative DNA damage implicated in diseases.
  • Antioxidants, particularly those forming coordination complexes with metal ions, are investigated for their protective potential against oxidative stress.
  • Understanding metal-mediated oxidative damage mechanisms is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the protective effects of selenium dioxide (SeO2) and sodium selenite (Na2SeO3) against transition metal-mediated oxidative DNA damage.
  • To elucidate the mechanisms by which selenium compounds mitigate both radical and non-radical oxidative DNA damage.
  • To assess the role of selenium-metal complexes in ROS production and their interaction with DNA.

Main Methods:

  • Assessing radical DNA damage by measuring 8-hydroxy-2'-deoxyguanosine (8-OH-dG) formation.
  • Evaluating non-radical DNA damage through ionic mechanisms involving adenine oxidation.
  • Utilizing fluorescence studies with dihydrodichlorofluorescein diacetate (DCF-DA) to quantify ROS production in various reaction systems.

Main Results:

  • Selenium compounds (SeO2, Na2SeO3) significantly reduced Fe(II)-, Cr(III)-, and Cu(II)-mediated radical DNA damage.
  • Pre-incubation of selenium compounds with metal ions before DNA addition enhanced protection, particularly for Fe(II) and Cr(III).
  • Non-radical adenine oxidation damage was also substantially decreased when selenium compounds interacted with metal ions first.
  • ROS production in selenium-metal-H2O2 systems (without DNA) was comparable to metal-H2O2 systems, indicating a sacrificial role for selenium complexes.

Conclusions:

  • Selenium dioxide and sodium selenite effectively inhibit transition metal-induced radical and non-radical oxidative DNA damage.
  • The protective effect is enhanced when selenium compounds pre-interact with metal ions, suggesting the formation of protective complexes.
  • Selenium-metal complexes appear to react sacrificially with H2O2, thereby preventing DNA oxidation and mitigating oxidative stress.