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Related Experiment Video

Updated: May 11, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Multi-analyte biochip (MAB) based on all-solid-state ion-selective electrodes (ASSISE) for physiological research.

Wan W Amani Wan Salim¹, Michael A Zeitchek, Andrew C Hermann

¹Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University. asalim@purdue.edu

Journal of Visualized Experiments : Jove

|May 1, 2013

Summary

This study introduces a multi-analyte biochip (MAB) using all-solid-state ion-selective electrodes (ASSISES) with a conductive polymer transducer for stable, long-term monitoring in complex media. The MAB successfully quantifies pH, carbonate, and calcium ions, demonstrating potential for environmental and biomedical applications.

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Last Updated: May 11, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

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Published on: April 18, 2013

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

Electrochemistry
Materials Science
Environmental Science

Background:

Lab-on-a-chip (LOC) applications require robust ion-selective electrodes (ISEs) for complex media.
All-solid-state ion-selective electrodes (ASSISES) offer advantages in miniaturization and ease of use.
Extending ASSISE lifetime necessitates stable potentials at interfacial layers.

Purpose of the Study:

To develop a microfabricated, all-solid-state ion-selective electrode (ASSISES) in a lab-on-a-chip format for multi-analyte sensing.
To enhance the working lifetime of ASSISES by utilizing conductive polymers (CPs) as transducer layers.
To demonstrate the capability of the developed multi-analyte biochip (MAB) for monitoring ions and biological activity.

Main Methods:

Fabrication of a microfabricated ASSISE (MAB) using a noble-metal electrode, a poly(3,4-ethylenedioxythiophene) (PEDOT) transducer layer, and an ion-selective membrane (ISM).
Electropolymerization of PEDOT films via cyclic voltammetry and galvanostatic deposition.
Spin-coating of ISMs and integration into a microfluidic flow-cell for measurements.
Calibration in test solutions and physiological measurements using the microalga Chlorella vulgaris.

Main Results:

The MAB successfully quantified H(+) (pH 4-9), CO3(2-) (0.01 mM - 1 mM), and Ca(2+) (0.01 mM to 1 mM).
The pH MAB exhibited a near-Nernstian response after one month of storage in algal medium.
The carbonate biochips showed potentiometric profiles comparable to conventional ISEs.
Physiological measurements demonstrated the MAB's ability to monitor biological activity.

Conclusions:

The developed multi-analyte biochip (MAB) offers a miniaturized, versatile platform for simultaneous sensing of multiple analytes.
The use of PEDOT as a transducer layer significantly extends ASSISE working lifetime and ensures stable interfacial potentials.
The MAB is suitable for confined monitoring situations in environmental, biomedical, and spaceflight research.