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Updated: May 11, 2026

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Recent progress in G-quadruplex DNA in deep eutectic solvent.

Chuanqi Zhao1, Xiaogang Qu

  • 1Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

Methods (San Diego, Calif.)
|May 1, 2013
PubMed
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Guanine-rich DNA forms G-quadruplex structures. This review explores their stability and dynamics in deep eutectic solvents (DES), offering a water-free medium for G-quadruplex DNA applications.

Area of Science:

  • Biochemistry and Supramolecular Chemistry
  • Nanotechnology and Medicinal Chemistry

Background:

  • Guanine-rich nucleic acids form G-quadruplex structures with significant applications.
  • Traditional DNA handling is limited to aqueous media, posing challenges for anhydrous reactions.
  • Deep eutectic solvents (DES) emerge as promising anhydrous media for DNA.

Purpose of the Study:

  • To review the stability, structure, folding dynamics, and thermodynamics of G-quadruplex DNA in DES.
  • To highlight the potential of DES as a medium for G-quadruplex research and applications.

Main Methods:

  • Spectroscopic techniques including circular dichroism (CD), UV-Vis spectroscopy, and fluorescence spectroscopy were employed.
  • Analysis of G-quadruplex behavior in various DES formulations.
Keywords:
Deep eutectic solventG-quadruplexParallel structureUltrastability

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Last Updated: May 11, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

Main Results:

  • G-quadruplex structures exhibit stability in DES.
  • DES can serve as a viable anhydrous medium for studying G-quadruplex folding and dynamics.
  • Spectroscopic data provide insights into G-quadruplex conformation and stability in DES.

Conclusions:

  • Deep eutectic solvents offer a novel and effective anhydrous environment for G-quadruplex DNA.
  • This opens new avenues for G-quadruplex applications in anhydrous conditions, relevant to chemistry and nanotechnology.