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Quality control, analysis and secure sharing of Luminex® immunoassay data using the open source LabKey Server
Josh Eckels1, Cory Nathe, Elizabeth K Nelson
1LabKey Software, Seattle, WA, USA.
BMC Bioinformatics
|May 2, 2013
Summary
LabKey Server enhances multiplexed bead assays for clinical trials by enabling custom data analysis and secure sharing. This open-source software facilitates high-throughput immunoassay data management and quality control.
Area of Science:
- Biotechnology
- Immunology
- Bioinformatics
Background:
- Multiplexed bead immunoassays generate high-content data crucial for evaluating humoral responses in clinical trials.
- Existing software often lacks integrated solutions for efficient data analysis, quality control, and secure sharing of this high-volume data.
- Enhanced software flexibility for customized analyses and visualizations is needed to maximize the utility of these assays in human clinical trials.
Purpose of the Study:
- To enhance the open-source LabKey Server platform for managing, analyzing, and sharing data from multiplexed bead immunoassays.
- To incorporate features that support custom data processing, quality control, and collaboration for Luminex assays.
Main Methods:
- Collaboration between the HIV Vaccine Trials Network, the Statistical Center for HIV/AIDS Research and Prevention, and LabKey Software.
- Enhancement of LabKey Server to support Luminex xMAP® technology-based multiplexed immunoassays.
- Implementation of features for data import, custom scripting (e.g., R), metadata collection, dose-response curve calculation, and quality control tracking (Levey-Jennings plots).
Main Results:
- LabKey Server now facilitates comprehensive workflows for multiplexed immunoassay data, including custom analysis and secure sharing.
- New features allow for flexible data import, script-based customization of analysis pipelines, and detailed quality control monitoring.
- The system supports titration analysis, concentration interpolation, data flagging, and outlier detection.
Conclusions:
- LabKey Server offers a unique, customizable solution for Luminex immunoassay analysis through scripting within a unified graphical interface.
- The platform excels in enabling secure data and analysis transfer among collaborators, distinguishing it from other Luminex tools.
- LabKey Server and its Luminex features are available as open-source software under the Apache 2.0 license.
