A simple shotgun proteomics method for rapid bacterial identification
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Updated: May 11, 2026

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
Published on: October 28, 2021
Dobryan M Tracz1, Stuart J McCorrister, Patrick M Chong
1National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
Abstract:
Bacterial pathogens were rapidly identified by shotgun proteomics using a novel, easy to implement database search strategy. Peptide sequence data from nano-LC-MS/MS was searched against a database represented by concatenated proteomes of completed genome sequences. Select bacterial species, including BSL-3 select agents, were used to demonstrate this method.
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