Related Experiment Video
Updated: May 11, 2026
06:01
Fluorescence Lifetime Macro Imager for Biomedical Applications
Published on: April 7, 2023
Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging
Shuna Cheng1, J Jesus Rico-Jimenez, Joey Jabbour
1Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA.
Optics Letters
|May 2, 2013
Summary
Multispectral fluorescence lifetime imaging (FLIM) aids tissue diagnosis. A new compact, time-gated FLIM endoscope enables simultaneous multi-band imaging for in vivo applications, overcoming previous limitations.
Area of Science:
- Biomedical Optics
- Medical Imaging Technology
- Endoscopic Instrumentation
Background:
- Fluorescence lifetime imaging (FLIM) provides noninvasive biochemical tissue analysis.
- Multispectral FLIM shows promise for medical diagnosis.
- Clinical application of FLIM is hindered by limitations in current endoscopy systems for in vivo imaging.
Purpose of the Study:
- To develop a compact and high-speed endoscopy system for multispectral FLIM.
- To overcome limitations of existing FLIM approaches for in vivo tissue imaging.
- To enable continuous lifetime imaging of multiple fluorescence emission bands.
Main Methods:
- Development of a compact, wide-field, time-gated FLIM flexible endoscope.
- Simultaneous imaging of up to three fluorescence emission bands.
Main Results:
- The developed endoscope is compact and capable of wide-field, time-gated FLIM.
- The system allows continuous lifetime imaging across three simultaneous fluorescence emission bands.
- This design addresses limitations of previous FLIM endoscopy systems.
Conclusions:
- The presented FLIM flexible endoscope is suitable for in vivo tissue imaging.
- This technology facilitates the evaluation of multispectral FLIM for clinical applications.
- The compact and high-speed design advances FLIM-based medical diagnosis.
