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Macromolecule extravasation-xenograft size matters: a systematic study using probe-based confocal laser
Antje Dietrich1, James Stewart, Melanie Huether
1Tumor Pathophysiology, OncoRay-National Center for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, Fetscherstraße 74, P.O. Box 41 , 01307, TU Dresden, Germany.
Molecular Imaging and Biology
|May 2, 2013
Summary
Tumor vascular changes impact drug delivery. This study developed a method to monitor tumor vasculature in xenografts, finding that tumor type and size significantly affect drug delivery and therapy outcomes.
Area of Science:
- Oncology
- Medical Imaging
- Vascular Biology
Background:
- Tumor vasculature undergoes significant changes impacting drug delivery and treatment efficacy.
- Subcutaneous xenograft models are crucial for preclinical therapy testing.
Purpose of the Study:
- To develop and validate a probe-based confocal laser endomicroscopy (pCLE) procedure for monitoring tumor vasculature.
- To assess morphological and functional vascular parameters in xenograft models relevant to drug delivery and therapy testing.
Main Methods:
- Established an experimental and analytical setup for systematic analysis of tracer extravasation from microvasculature.
- Applied probe-based confocal laser endomicroscopy (pCLE) in HCT-116 and SW620 xenograft models throughout tumor growth.
Main Results:
- Increased incidence of dilated vessels observed with growing xenograft size in both models.
- Macromolecule extravasation and tracer accumulation in tumor tissue significantly decreased with tumor growth.
- Correlation between dilated vessels and tracer extravasation varied between HCT-116 and SW620 models.
Conclusions:
- Tumor xenograft type and size are critical determinants for effective drug delivery.
- Findings highlight the importance of considering vascular characteristics in xenograft models for therapy testing.
