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Related Experiment Video

Updated: May 11, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

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Published on: September 5, 2013

Associations between dru Types and SCCmec cassettes.

Mette D Bartels1, Kit Boye, Duarte C Oliveira

  • 1Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark. mette.damkjaer@dadlnet.dk

Plos One
|May 2, 2013
PubMed
Summary
This summary is machine-generated.

The direct repeat units (dru) region shows limited diversity in methicillin-resistant Staphylococcus aureus (MRSA) isolates, suggesting it is not ideal for initial outbreak investigations. However, dru typing can track MRSA evolution within individual patients.

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Area of Science:

  • Microbiology
  • Molecular Epidemiology
  • Genetics

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) outbreaks require effective molecular typing for investigation and monitoring.
  • The staphylococcal cassette chromosome mec (SCCmec) harbors a variable direct repeat units (dru) region, proposed for supplementary MRSA typing.
  • An international nomenclature for the dru region exists, facilitating standardized analysis.

Purpose of the Study:

  • To investigate the diversity and variability of the dru region within a diverse collection of MRSA isolates.
  • To assess the utility of dru typing as a supplementary method for MRSA characterization.
  • To explore the relationship between dru types, SCCmec types, and overall MRSA genetic background.

Main Methods:

  • Analysis of 302 MRSA isolates harboring SCCmec types I-VI.
  • Utilized Staphylococcal protein A (spa) typing and multilocus sequence typing (MLST) for genetic background assessment.
  • Sequencing of the dru region to identify diversity and variability.

Main Results:

  • 53 distinct dru types (dt) were identified in 283 isolates; 18 lacked dru repeats.
  • The most prevalent dru type, dt10a (53%), was found across multiple SCCmec types (except II).
  • Dru type variants were observed in 10% of epidemiologically linked patients, but isolates from single patients over time showed consistent dru types.

Conclusions:

  • The dru region exhibits limited diversity, making it unsuitable as a primary epidemiological typing tool for MRSA outbreaks.
  • Dru typing may be valuable for tracking the evolution of MRSA within individual patients over extended periods.
  • The widespread presence of dt10a across various SCCmec types suggests a common origin for dru and the mecA gene in Staphylococcus species.