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Gamma-tocotrienol modulated gene expression in senescent human diploid fibroblasts as revealed by microarray analysis
Suzana Makpol1, Azalina Zainuddin, Kien Hui Chua
1Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. suzanamakpol@yahoo.com
Oxidative Medicine and Cellular Longevity
|May 2, 2013
Summary
Gamma-tocotrienol, a vitamin E form, impacts gene expression in aging human cells. This compound may prevent cellular aging by modulating key biological processes in fibroblasts.
Area of Science:
- Biochemistry
- Cell Biology
- Gerontology
Background:
- Cellular aging, or senescence, is characterized by altered gene expression.
- Vitamin E isomers, like gamma-tocotrienol, are investigated for their biological effects.
Purpose of the Study:
- To investigate the effect of gamma-tocotrienol on gene expression in senescent human diploid fibroblasts.
- To determine if gamma-tocotrienol can modulate cellular aging processes.
Main Methods:
- Senescent human diploid fibroblasts were treated with 70 μM gamma-tocotrienol for 24 hours.
- Gene expression analysis was performed using Illumina BeadChip arrays and quantitative RT-PCR.
- Gene Set Enrichment Analysis (GSEA) was used to identify modulated biological pathways.
Main Results:
- Gamma-tocotrienol treatment resulted in differential expression of 100 genes (P < 0.001) by at least 1.5 fold.
- Key genes modulated include IRAK3, SelS, HSPA5, and HERPUD1.
- GSEA indicated modulation of inflammation, protein transport, apoptosis, and cell redox homeostasis.
Conclusions:
- Gamma-tocotrienol significantly alters gene expression patterns in senescent human fibroblasts.
- The findings suggest gamma-tocotrienol possesses anti-aging properties by modulating critical cellular pathways.
- Gamma-tocotrienol shows potential in preventing cellular aging through gene expression modulation.
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