Related Experiment Videos
Intercomparison of small biomolecules by gel filtration and small angle scattering
P Martel1, T M Stepanik, A G Szabo
1Atomic Energy of Canada Limited, Chalk River Nuclear Laboratories, Ontario, Canada.
Analytical Biochemistry
|May 1, 1990
Abstract:
This paper describes how small angle neutron scattering (SANS) can be used to confirm that gel filtration results are free of dimerization effects. After characterization by analytical gel filtration, concentrated solutions (in heavy water, D2O) of a cobra neurotoxin, a cytotoxin, and a cytotoxin analog are studied by SANS. Small differences in shape are shown to be discernible by means of least-square fits to ellipsoidal models. The parallel axis theorem is then invoked to assess dimerization levels statistically. The results are briefly discussed on the basis of function in relation to structure.