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Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
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Related Experiment Video

Updated: May 11, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

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Published on: February 12, 2022

A nexus for receptor recycling.

Suzanne R Pfeffer1

  • 1Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA. pfeffer@stanford.edu

Nature Cell Biology
|May 3, 2013
PubMed
Summary
This summary is machine-generated.

Sorting nexin 27 (SNX27) and the retromer complex are crucial for recycling cell surface proteins. This study identifies specific proteins dependent on SNX27 and retromer for maintaining their cell surface location.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Proteomics

Background:

  • Sorting nexin proteins (SNXs) and the retromer complex are essential for intracellular trafficking.
  • Receptor recycling from endosomes to the cell surface is a critical process for cellular function.
  • SNX27 is known to interact with various cargo proteins.

Purpose of the Study:

  • To identify cell surface proteins whose localization depends on SNX27 and the retromer complex.
  • To understand the role of SNX27 and retromer in maintaining steady-state cell surface protein levels.
  • To provide a comprehensive proteomic view of SNX27-retromer-dependent cell surface proteins.

Main Methods:

  • Global proteomics analysis
  • Endosomal sorting and trafficking studies
  • Protein localization assays

Main Results:

  • A specific set of cell surface proteins was identified.
  • These proteins were found to rely on SNX27 and the retromer complex for their cell surface localization.
  • The study highlights the importance of this pathway for maintaining protein homeostasis at the cell surface.

Conclusions:

  • SNX27 and the retromer complex are critical regulators of cell surface protein homeostasis.
  • Disruption of SNX27-retromer function leads to altered cell surface proteomes.
  • This work provides new insights into the molecular mechanisms governing receptor recycling.