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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
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Related Experiment Video

Updated: May 11, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Decoding split and pool combinatorial libraries with electron-transfer dissociation tandem mass spectrometry.

Mohosin Sarkar1, Bruce D Pascal, Caitlin Steckler

  • 1Department of Chemistry, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA.

Journal of the American Society for Mass Spectrometry
|May 3, 2013
PubMed
Summary
This summary is machine-generated.

We developed a new mass spectrometry method using electron-transfer dissociation (ETD) to decode complex chemical libraries. This approach successfully decodes chiral oligomers of pentenoic amides (COPAs), enabling drug discovery.

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Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
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Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

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Last Updated: May 11, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

Area of Science:

  • Chemical biology
  • Mass spectrometry
  • Drug discovery

Background:

  • Bead-based split and pool combinatorial chemistry enables large, diverse chemical library synthesis.
  • Decoding the sequence of screening hits is crucial for identifying novel compounds.
  • Existing methods struggle with sequence-specific decoding of certain oligomers.

Purpose of the Study:

  • To develop and validate an electron-transfer dissociation (ETD) based tandem mass spectrometry approach for decoding mass-encoded split and pool libraries.
  • To demonstrate the efficacy of ETD for sequencing chiral oligomers of pentenoic amides (COPAs).
  • To introduce tools for aiding the decoding process and library generation.

Main Methods:

  • Utilized electron-transfer dissociation (ETD) tandem mass spectrometry for sequence decoding.
  • Synthesized and analyzed chiral oligomers of pentenoic amides (COPAs).
  • Incorporated bromine (Br) isotope tags for differentiating N- and C-terminal ions.
  • Developed the "Hit-Find" software for in silico library generation and searching.

Main Results:

  • ETD successfully provided complete sequence information for COPAs, unlike collisional activated dissociation.
  • Bromine isotope tags effectively differentiated N- and C-terminal product ions.
  • "Hit-Find" software facilitates in silico library analysis within a defined mass error.

Conclusions:

  • ETD-based mass spectrometry is a powerful tool for decoding complex, mass-encoded chemical libraries.
  • This method significantly advances the identification of novel bioactive compounds from split and pool libraries.
  • The developed tools enhance the efficiency of drug discovery pipelines.