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Identification of DNA ligase I related polypeptides in three different human cells
C Prigent1, S Aoufouchi, M Philippe
1Laboratoire de Biologie et Génétique du Développement URA CNRS 256, Université de Rennes I, France.
Biochemical and Biophysical Research Communications
|June 29, 1990
Abstract:
Partial purification of the DNA ligase from three human tissues (liver, thymus and lymphoblasts) revealed that each cell type contains several different polypeptides bearing a DNA ligase I activity. Their apparent molecular weights estimated after SDS PAGE, 130 kDa, 100 kDa and 80 kDa, are in agreement with previous reports. These polypeptides are related by proteolysis to a single higher molecular weight protein of 200 kDa which does not show DNA ligase activity but that could be a preprotein.