Related Experiment Video
Updated: May 11, 2026
08:43
Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
Published on: June 19, 2018
Measuring vascular permeability in mice
Cold Spring Harbor Protocols
|May 3, 2013
Summary
This study presents noninvasive methods to measure vascular permeability in mice using fluorescent tracers and microscopy. These techniques assess both average and individual vessel permeability in normal and diseased tissues.
Area of Science:
- Vascular biology
- Biomedical imaging
- Microscopy techniques
Background:
- Assessing vascular parameters is crucial for understanding tissue physiology and disease.
- Noninvasive methods are needed for in vivo studies of vascular function.
- Vascular permeability is a key parameter affected in various diseases.
Purpose of the Study:
- To develop and present noninvasive protocols for measuring vascular permeability in mice.
- To enable the assessment of average and individual vessel permeability.
- To provide a method applicable to both normal and diseased tissues.
Main Methods:
- Injection of fluorescent tracers into mice.
- Measurement of average vascular permeability (P) using single-photon microscopy.
- High-resolution measurement of individual vessel segment permeability using multiphoton laser-scanning microscopy (MPLSM) near tumor/window interfaces.
Main Results:
- Demonstration of noninvasive techniques for vascular permeability assessment.
- Capability to measure both average and spatially resolved vascular permeability.
- Protocol applicable to mouse models of normal and diseased tissues.
Conclusions:
- Noninvasive microscopy techniques allow for robust assessment of vascular permeability in mice.
- The described methods provide valuable tools for studying vascular function in vivo.
- These techniques can be applied to research on various vascular-related diseases.
