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Updated: May 11, 2026
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Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Published on: June 29, 2020
Comparative analysis of routine laboratory diagnostic tests for rabies
S S Kadam1, A A Sherikar, V S Pingale
1Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 MS India.
Summary
This study compared laboratory diagnostic tests for rabies detection. Counter immunoelectrophoresis test (CIET) proved most sensitive and rapid for diagnosing rabies virus.
Area of Science:
- Veterinary Virology
- Diagnostic Pathology
Background:
- Rabies diagnosis relies on various laboratory techniques.
- Accurate and rapid diagnostic methods are crucial for disease control.
Purpose of the Study:
- To compare the diagnostic performance of routine laboratory tests for rabies.
- To identify the most sensitive and rapid method for rabies detection.
Main Methods:
- Evaluated Seller's staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT), and counter immunoelectrophoresis test (CIET).
- Tested 200 brain specimens for rabies virus detection.
Main Results:
- Seller's staining identified Negri bodies in 52 samples.
- MIT isolated rabies virus in 56 samples.
- Dot-ELISA and AGPT detected antigen in 55 and 57 samples, respectively.
- CIET demonstrated the highest sensitivity and speed among the tested methods.
Conclusions:
- Counter immunoelectrophoresis test (CIET) is the superior diagnostic method for rabies.
- CIET offers a sensitive and rapid approach for laboratory diagnosis of rabies.
