Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

Genome Size and the Evolution of New Genes

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.

External Anatomy of the Kidney

External Anatomy of the Kidney

The kidneys are a pair of bean-shaped organs in the human body that play a critical role in maintaining overall health. They filter out waste products from the blood, regulate blood pressure, maintain electrolyte balance, and stimulate the production of red blood cells.
The kidneys are located in the retroperitoneal space on either side of the vertebral column, protected posteriorly by the 11th and 12th ribs. The right kidney sits slightly lower than the left owing to the presence of the liver...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Developing And Internally Validating AI-Based Aging Resilience Biomarkers in Non-Human Primates.

bioRxiv : the preprint server for biology·2026

Same author

Developing and internally validating AI-based aging resilience biomarkers in non-human primates.

The journals of gerontology. Series A, Biological sciences and medical sciences·2026

Same author

Identification of putative kidney-derived proteins in plasma using nanoparticle enrichment.

Molecular omics·2026

Same author

MicroRNA Regulatory Targets Related to VO<sub>2</sub>peak Decline in Older Adult Participants of the Study of Muscle, Mobility and Aging (SOMMA).

bioRxiv : the preprint server for biology·2026

Same author

T-Cell Signaling Pathways, Including Exhaustion, Predominate in Unhealthy Visceral and Subcutaneous Adipose Tissues.

Obesity (Silver Spring, Md.)·2026

Same author

Prenatal glucocorticoid exposure programs long-term left ventricular metabolism, function, and remodeling in baboons.

American journal of physiology. Heart and circulatory physiology·2026

Search research articles

Related Experiment Video

Updated: May 11, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

The baboon kidney transcriptome: analysis of transcript sequence, splice variants, and abundance.

Kimberly D Spradling¹, Jeremy P Glenn, Roy Garcia

¹Department of Genetics, Texas Biomedical Research Institute, San Antonio, Texas, United States of America. kspradli@txbiomedgenetics.org

|May 3, 2013

Summary

This study characterizes the baboon kidney transcriptome, identifying thousands of novel genes and genetic variations. This provides a crucial genomic resource for using baboons in biomedical research, particularly for kidney disease studies.

More Related Videos

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Effective Rapid Blood Perfusion in Xenopus

Effective Rapid Blood Perfusion in Xenopus

Published on: May 16, 2023

Related Experiment Videos

Last Updated: May 11, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Effective Rapid Blood Perfusion in Xenopus

Effective Rapid Blood Perfusion in Xenopus

Published on: May 16, 2023

Area of Science:

Genomics
Comparative Genomics
Transcriptomics

Background:

Baboons are vital models for human disease research, especially kidney diseases.
A lack of comprehensive genomic resources hinders baboon model utility.
Genomic data is essential for advancing biomedical research using baboons.

Purpose of the Study:

To characterize the baboon kidney transcriptome.
To identify genes, variants, and pathways in the baboon kidney.
To establish a valuable genomic resource for baboon research.

Main Methods:

High-throughput cDNA sequencing (RNA-Seq) of baboon kidney tissue.
Comparison of baboon cDNA sequences with the human hg18 reference assembly.
Gene ontology analysis to identify biological functions and pathways.

Main Results:

Identified 35,900 cDNAs, including 35,150 novel transcripts representing 15,369 genes.
Discovered 45,499 high-confidence single nucleotide polymorphisms (SNPs) and 29,813 insertion-deletion polymorphisms (InDels).
Revealed significant metabolic pathways and biological functions relevant to kidney function.

Conclusions:

The study catalogues transcribed elements in the baboon kidney.
A comprehensive genomic resource for the baboon kidney has been established.
This resource will enhance the use of baboons in biomedical and kidney disease research.