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Related Experiment Video

Updated: May 11, 2026

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

Defining the subcellular interface of nanoparticles by live-cell imaging.

Peter H Hemmerich1, Anna H von Mikecz

  • 1Leibniz-Institute for Age Research, Fritz-Lipman-Institute, Jena, Germany. mikecz@tec-source.de

Plos One
|May 3, 2013
PubMed
Summary

This study presents advanced fluorescence microscopy techniques to track nanoparticle behavior inside cells. We reveal how nanoparticles enter, move within, and interact with cellular components, aiding in the development of intracellular delivery systems.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Understanding nanoparticle-cell interactions is crucial for developing targeted drug delivery systems.
  • The dynamic behavior of nanoparticles within cells, including uptake and organelle interactions, remains incompletely understood.

Purpose of the Study:

  • To develop and present a combined kinetic imaging protocol for investigating nanoparticle intracellular fate.
  • To analyze the dynamic behavior and biophysical properties of nanoparticles within living cells.

Main Methods:

  • Time-lapse confocal microscopy for observing nanoparticle uptake.
  • Fluorescence recovery after photobleaching (FRAP) to study nanoparticle exchange at organelles.
  • Fluorescence correlation spectroscopy (FCS) and raster image correlation spectroscopy (RICS) for diffusion analysis.

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Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles
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Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

Published on: November 1, 2017

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Last Updated: May 11, 2026

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles
10:26

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

Published on: November 1, 2017

Main Results:

  • Fast, dynamin-dependent cellular uptake of polystyrene and silica nanoparticles observed.
  • Rapid and complete nanoparticle exchange at mitochondria, vesicles, and the nuclear envelope.
  • Nanoparticles translocate into the nucleus via diffusion and active transport within minutes.

Conclusions:

  • The presented fluorescence fluctuation microscopy toolkit enables detailed investigation of nanomaterial biophysics in subcellular environments.
  • Nanoparticles function as mobile, monodisperse entities within the cytoplasm and nucleus.
  • This work contributes to a framework for understanding intracellular nanoparticle delivery routes.