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Updated: May 11, 2026

Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
Published on: October 2, 2010
Coronatine inhibits stomatal closure and delays hypersensitive response cell death induced by nonhost bacterial
Seonghee Lee1, Yasuhiro Ishiga, Kristen Clermont
1The Samuel Roberts Noble Foundation , Plant Biology , Ardmore, Oklahoma , USA.
Abstract:
Pseudomonas syringae is the most widespread bacterial pathogen in plants. Several strains of P. syringae produce a phytotoxin, coronatine (COR), which acts as a jasmonic acid mimic and inhibits plant defense responses and contributes to disease symptom development. In this study, we found that COR inhibits early defense responses during nonhost disease resistance. Stomatal closure induced by a nonhost pathogen, P. syringae pv. tabaci, was disrupted by COR in tomato epidermal peels. In addition, nonhost HR cell death triggered by P. syringae pv. tabaci on tomato was remarkably delayed when COR was supplemented along with P. syringae pv. tabaci inoculation. Using isochorismate synthase (ICS)-silenced tomato plants and transcript profiles of genes in SA- and JA-related defense pathways, we show that COR suppresses SA-mediated defense during nonhost resistance.
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