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Published on: July 11, 2015
Heat shock induces interferon-TAU gene expression by in vitro-produced bovine blastocysts
Cristina F Hickman1, Michael Clinton, Alison Ainslie
1SRUC, Edinburgh, UK.
Problem:
The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro.
Method Of Study:
In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified.
Results:
Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not.
Conclusion:
The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.
