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Laser phototherapy at high energy densities do not stimulate pre-osteoblast growth and differentiation
Pedro Silva Pacheco1, Flávia Amadeu de Oliveira, Rodrigo Cardoso Oliveira
1Bauru School of Dentistry, Bauru, SP, Brasil.
Objective:
The aim of this study is to evaluate the effects of red and infrared lasers at high energy densities on pre-osteoblast MC3T3 proliferation and differentiation.
Background Data:
The acceleration of bone regeneration by low intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry.
Materials And Methods:
Cells were irradiated with red (660 nm) and infrared (780 nm) lasers (90 and 150 J/cm2, 40 mW). The control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, 96 h) and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72, 96 h; 7, 14 days).
Results:
None of the irradiation groups had an enhancement in cell growth (p<0.05). The production of ALP was not influenced by irradiation at any period of time (p>0.05).
Conclusions:
The low intensity laser stimulated neither cell growth nor the production of alkaline phosphatase.
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