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Updated: May 11, 2026
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
Published on: February 6, 2017
[Comparison of two nucleic acid extraction methods for norovirus in oysters]
Qiao Yuan1, Hui Li, Xiaoling Deng
1Sun Yat-Sen University School of Public Health, Guangzhou 510080, China. yuanrachel@sohu.com
Objective:
To explore a convenient and effective method for norovirus nucleic acid extraction from oysters suitable for long-term viral surveillance.
Methods:
Two methods, namely method A (glycine washing and polyethylene glycol precipitation of the virus followed by silica gel centrifugal column) and method B (protease K digestion followed by application of paramagnetic silicon) were compared for their performance in norovirus nucleic acid extraction from oysters. Real-time RT-PCR was used to detect norovirus in naturally infected oysters and in oysters with induced infection.
Results:
The two methods yielded comparable positive detection rates for the samples, but the recovery rate of the virus was higher with method B than with method A.
Conclusion:
Method B is a more convenient and rapid method for norovirus nucleic acid extraction from oysters and suitable for long-term surveillance of norovirus.
