Enoxacin inhibits growth of prostate cancer cells and effectively restores microRNA processing

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Prostate cancer (PCa) is a prevalent malignancy with limited treatment options for advanced stages.
• MicroRNA (miRNA) dysregulation, particularly downregulation, is implicated in PCa development and progression.
• Restoring global miRNA expression is a potential therapeutic strategy for PCa.

Purpose of the Study:

• To evaluate enoxacin as an anti-tumoral agent for PCa.
• To investigate enoxacin's ability to induce miRNA biogenesis via TARBP2 (TRBP).
• To assess enoxacin's effects on PCa cell viability, apoptosis, cell cycle, and invasiveness.

Main Methods:

• Utilized five PCa cell lines and primary prostate carcinoma samples.
• Assessed TARBP2 gene status and TRBP protein expression.
• Administered enoxacin and evaluated its impact on cell viability, apoptosis, cell cycle arrest, invasiveness, and global miRNA expression.

Main Results:

• PCa cell lines expressed wild-type TARBP2 and TRBP protein.
• Enoxacin significantly decreased PCa cell viability and invasiveness.
• Enoxacin induced apoptosis and cell cycle arrest in PCa cells.
• Enoxacin effectively restored global miRNA expression in PCa cells.

Conclusions:

• PCa cells are highly responsive to enoxacin's anti-tumoral effects.
• Enoxacin demonstrates potential as a therapeutic agent for prostate cancer by restoring miRNA biogenesis.
• Enoxacin's ability to modulate miRNA expression and inhibit tumor progression warrants further investigation.